Search for immunoregulatory factors derived from LSECs and Cancer cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24510300
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Kyushu University
• Principal Investigator: KANO Arihiro 九州大学, 先導物質化学研究所, 准教授 (30403950)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: がん / 腫瘍 / 免疫抑制 / IFN-γ

Outline of Final Research Achievements

Based on the production of IFN-γ from the cultivated splenocytes, I aimed to identify immunoregulatory factors from liver sinusoidal endothelial cells (LSEC) and cancer cells. I succeeded to isolate and purify LSEC in more than 90% with magnet beads. I discovered that the production of IFN-γin the cultured splenocytes from the 4T1 tumor-bearing mouse is transiently upregulated and then decreased, and further suppressed by 4T1-conditioned medium. The purification of the suppressive factors from the 4T1-conditioned medium succeeded in the identification of G-CSF and M-CSF. The suppressive factors in the CT26-conditioned medium seemed to be smaller than 10kDa, therefor; these were analyzed by HPLC and LC-MS and succeeded in to show PGE2 in the conditioned medium. It is hoped that various immunosuppressive substances are identified from this assay system.

Free Research Field

分子細胞生物学