The study of light-induced structural change and DNA binding formation in Aureochrome with NMR

2014 Fiscal Year Final Research Report

• Project/Area Number: 24540435
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biophysics/Chemical physics
• Research Institution: Osaka University
• Principal Investigator: HAMADA Norio 大阪大学, 産学連携本部, 講師 (80379148)
• Co-Investigator(Kenkyū-buntansha): HISATOMI Osamu 大阪大学, 大学院理学研究科, 准教授 (60231544)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: NMR / 構造変化 / 光受容タンパク質 / DNA結合 / 転写因子

Outline of Final Research Achievements

Aureochrome-1 is a blue light receptor that mediated the branching response in stramenopile alga and light-regulated transcription factor, although molecular mechanism is unknown. We have tried to measure light-induced structural change and DNA binding formation in Aureochrome with NMR spectroscopy. Light-induced structural change signals were observed in photo-steady state of Aureochrome with NMR. We confirmed the different peaks between dark state and photo-steady state in Aureochrome. However some peaks were not assigned in photo-steady state due to signal broadening. To obtain the structural information, we produced mutants according to similar LOV domain sequences. We found that some mutants were stabilized photo-steady conformation.

Free Research Field

生物物理