Multinuclear Dynamic NMR of Diffusion and Transport of Popypeptides by Membrane Thermal Fluctuation

2015 Fiscal Year Final Research Report

• Project/Area Number: 24550035
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical chemistry
• Research Institution: Himeji Dokkyo University
• Principal Investigator: Okamura Emiko 姫路獨協大学, 薬学部, 教授 (00160705)
• Co-Investigator(Kenkyū-buntansha): AKI KENZO 姫路獨協大学, 薬学部, 助手 (50714945) ; TAKECHI YUKI (HARAYA YUKI) 姫路獨協大学, 薬学部, 助教 (30634604)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 生物物理 / 界面・表面物性 / 超精密解析 / NMR / 膜輸送

Outline of Final Research Achievements

Molecular dynamics in membrane inside and outside was quantified by multinuclear, dynamic NMR in situ, in relation to the thermal fluctuation of membranes. First, the lipid membrane dynamics was successfully characterized in cell sized vesicles (CSVs) of 10-20 μm-diameters by using solution-state NMR. The dynamic structure of CSVs was found to be different from nano-sized vesicles of ~100 nm-diameters frequently used. The contribution of membrane fluctuation to molecular transport was also quantified in the presence of various amounts of cholesterol in the membrane.
The peptide permeation to living cell inside was also observed by real-time in-cell NMR in situ and analyzed by first-order kinetics. Such kinetic approach was also applicable to time-resolved studies of biochemical reactions in peptides such as peptide bond cleavage in human-lens crystallin fragment in situ.

Free Research Field

生物物理化学