2014 Fiscal Year Final Research Report
Photoinduced electron- and energy-transfer reactions of protein-photosensitizer complexes
| Project/Area Number |
24550078
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Inorganic chemistry
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| Research Institution | Nara Women's University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 光 / 電子移動 / ルテニウム / キモトリプシン / カルボニックアンヒドラーゼ / 金属錯体 / DFT計算 |
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| Outline of Final Research Achievements |
By using preferential enzyme-inhibitor interactions, we study the photophysical properties of artificial Carbonic Anhydrase or Chymotrypsin-[Ru(bpy)3]2+ type complex and the detailed photoinduced electron- or energy-transfer mechanisms are discussed. For example, we prepared a Ru(II)-based CA inhibitor,tethering a benzenesulfonamide group and a [Ru(bpy)3]2+ moiety and constructed the artificial CA-Ru(II) complex. After steady-state photoirradiation, the photo-excited triplet state of Ru(II)* was quenched by a sacrificial quencher through an intermolecular photoinduced ET mechanism, giving the oxidized Ru(III) complex. The following intramolecular electron abstraction from an amino-acid residue near the active site of CA proceeded.
On the other hand, several types of the [Ru(bpy)2(L)]2+ complexes were synthesized and their electrochemical and spectral properties were examined. Spin densities were analyzed to characterize triplet states in those complexes.
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| Free Research Field |
生物無機化学
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