2014 Fiscal Year Final Research Report
Development of Highly-sensitive Analytical Technique Using Bipolar Electrodes in Microchip Electrophoresis of Biomolecules
| Project/Area Number |
24550090
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 分析科学 / マイクロ・ナノデバイス |
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| Outline of Final Research Achievements |
In this study, to increase the detection sensitivity in capillary electrophoresis (CE) and microchip electrophoresis (MCE), a novel and simple on-line sample preconcentration based on a modified dynamic pH junction technique in a bipolar electrode embedded single “straight” channel was developed. It was found that a very high pH zone with a short band width formed around the bipolar electrode only by switching the polarity of the separation voltage applied both ends of the straight microchannel. As a typical result, a 50～300-fold increases in the sensitivity for fluorescent dyes were obtained. Proposed technique was successfully applied to the analysis of labeling dyes for biomolecules and the separation of protein mixtures.
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| Free Research Field |
分析化学
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