Real-time observation of metal nanoparticles internalized into a biological cell by confocal microscopy

2014 Fiscal Year Final Research Report

• Project/Area Number: 24550091
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Akita University
• Principal Investigator: FUJIWARA Kazuhiko 秋田大学, 工学(系)研究科(研究院), 准教授 (10375222)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 金ナノ粒子 / 局在表面プラズモン共鳴 / 光散乱 / 顕微鏡

Outline of Final Research Achievements

Analyses of protein localization and interactions in biological cells are very important on tumor marker targeted medical diagnostics and profiling of some series of diseases. Nanoparticles receive an enormous attention in various biomedical applications since their sizes are similar with biological molecules and structures. Gold nanoparticle (AuNP) possesses unique optical properties based on localized surface plasmon resonance (LSPR) and functionalizability with self-assembled monolayers, thus they have utilized in biosensing and gene delivery applications.
We studied to develop a light-scattering microscopy to probe AuNP internalized into biological cells. The optimum condition for surface modification of AuNPs and intracellular introduction by protein coated AuNP were investigated. Resonant light scattering imaging of AuNPs in a cell was also demonstrated in this study. Cellular uptake and fate of AuNPs were analyzed.

Free Research Field

分析化学