Flow-based multi-biosensing system fabricated with track-etched microporous membrane electrodes

2014 Fiscal Year Final Research Report

• Project/Area Number: 24550092
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Yamagata University
• Principal Investigator: MIZUGUCHI HITOSHI 山形大学, 理工学研究科, 助教 (30333991)
• Research Collaborator: KIJIMA Tatsuro 山形大学, 大学院理工学研究科, 准教授 (50272084) ; TACHIBANA Kazuhiro 山形大学, 大学院理工学研究科, 准教授 (50241724) ; NISHINA Tatsuo 山形大学, 大学院理工学研究科, 教授 (60172673) ; IIYAMA Masamitsu 野村マイクロサイエンス株式会社
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: バイオセンサー / 電気分析 / 酵素 / トラックエッチドメンブレン / フロー分析

Outline of Final Research Achievements

The new amperometric biosensor was fabricated by using track-etched microporous membrane electrodes. The proposed sensor system consists a prereactor electrode, a detector electrode, and an immobilized enzyme reactor sandwiched between these electrodes. The interferents such as L-ascorbic acid or uric acid are electrolyzed completely at the prereactor electrode, which is positioned upstream of the enzyme reactor. The increased current response at the detector electrode derives solely from oxidation of hydrogen peroxide produced in the enzymatic reaction. Sensitive and selective response toward analyte concentration was obtained with no other catalytic material such as peroxidase or an electron mediator.

Free Research Field

分析化学