Development of highly effective synthesis methods for tumor and disease specific carbohydrates

2014 Fiscal Year Final Research Report

• Project/Area Number: 24550134
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Polymer chemistry
• Research Institution: Kyoto University
• Principal Investigator: OHMAE Masashi 京都大学, 工学(系)研究科(研究院), 助教 (50300801)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 癌関連糖鎖抗原 / 糖鎖有機合成 / 糖加水分解酵素 / 硫酸化糖鎖 / 酵素触媒重合

Outline of Final Research Achievements

This study was carried out to construct effective synthesis methods for disease-related carbohydrates, leading to investigation of their structure-activity relationships. Particularly, tumor-associated type II sulfated carbohydrate antigens were the major synthesis targets in this study. This type of antigen has L-fucose branches, consisting of complex sulfated Lewis X units in a molecular chain. We first established a facile and rapid synthesis method for a sulfated Lewis X unit. We further found the novel methodology to obtain sulfated type II carbohydrate antigens containing L-fucose branches by the catalysis of keratanase II, which can transfer the sulfated Lewis X oxazoline derivative to the other type II sulfated chain. These important findings opened up the way to the synthesis of complex type II sulfated carbohydrate antigens for the first time.

Free Research Field

糖鎖工学