2014 Fiscal Year Final Research Report
Site direct mutagenesis of multiheme protein
| Project/Area Number |
24550183
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
KAMACHI Toshiaki 東京工業大学, 生命理工学研究科, 准教授 (30272694)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 電子伝達タンパク質 |
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| Outline of Final Research Achievements |
Site directed mutagenesis of cytochrome c3 from Desulfovibrio vulgaris was carried out for the addition of enzymatic activity to the electron transfer protein. Cytochrome c3 was tetraheme electron transfer protein and our previous study reveals the role of each heme. one of the heme plays an important role in electron donation to physiological electron acceptor, hydrogenase. So in this study, heme coordination sphere of the heme was changed by the site direct mutagenesis. Obtained cytochrome c3 can carried out both enzymatic activity and electron transfer activity.
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| Free Research Field |
生物無機化学
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