Development of Rapid and Sensitive Fluorescent Probes for Hydrogen Peroxide Bio-imaging

2014 Fiscal Year Final Research Report

• Project/Area Number: 24550197
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Doshisha University
• Principal Investigator: HITOMI Yutaka 同志社大学, 理工学部, 教授 (20335186)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: イメージング / 蛍光 / 過酸化水素 / 検出 / 細胞

Outline of Final Research Achievements

A series of fluorescent probes for hydrogen peroxide have been developed that have a mononuclear nonheme iron complex as a reaction site toward hydrogen peroxide. They are all non-fluorescent, but once reacting with low concentrations of hydrogen peroxide inside cells, they release green- or red-emitting fluorescent dyes such as resorufin and fluorescein. We have succeeded in real-time imaging of hydrogen peroxide that was produced as a messenger inside A431 cells upon stimulation of epidermal growth factors.

Free Research Field

生体関連化学