2014 Fiscal Year Final Research Report
Recognition of metal-mediated base pairs by DNA polymerases
| Project/Area Number |
24550199
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Osaka University of Pharmaceutical Sciences |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 金属錯体型塩基対 / DNAポリメラーゼ / プライマー伸長反応 / 銀(I)イオン |
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| Outline of Final Research Achievements |
In this project, first we studied the DNA polymerase-catalyzed primer extension in the presence of Ag(I) ions and unexpectedly found the formation of not the C-Ag(I)-C base pair but the C-Ag(I)-A base pair. Subsequently, we found the formation of the C-Ag(I)-T base pair by exhaustive screening of DNA polymerase-catalyzed mismatch base pair formation under the presence of various kinds of metal ions. By using very high specificity of the formation of the C-Ag(I)-T base pair and the previously reported T-Hg(II)-T base pair, we demonstrated that two kinds of metal ions, Ag(I) and Hg(II), highly selectively mediate the incorporation of thymidine 5’-triphosphate (dTTP) into the sites opposite cytosine and thymine in the template, respectively. In other words, the regulated incorporation of different metal ions into the programmed sites in duplex by DNA polymerase was successfully achieved.
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| Free Research Field |
核酸化学
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