Development of PLLA Nanofiber Scaffold Doped with BaTiO3 by Electrospinning for Bone/Cartilage Tissue Engineering

2014 Fiscal Year Final Research Report

• Project/Area Number: 24560114
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Materials/Mechanics of materials
• Research Institution: Doshisha University
• Principal Investigator: MORITA Yusuke 同志社大学, 生命医科学部, 教授 (80368141)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 骨軟骨再生 / スキャホールド / ナノファイバー / チタン酸バリウム粒子 / エレクトロスピニング

Outline of Final Research Achievements

In this study, PLLA nanofiber scaffold doped with BaTiO3 (BTO) particle was developed to accelerate bone formation. We developed PLLA/BTO nanofiber scaffold with uniformly dispersed BTO nanoparticles at PLLA concentration of 10 w/v% and BTEAC concentration of 3 w/v%. According to TEM observation, BTO nanoparticles were dispersed uniformly in the PLLA/BTO nanofiber, and the density of BTO nanoparticle in the PLLA/BTO nanofiber increased with increasing amount of BTO. The surface potential of PLLA/BTO nanofiber was lower than that of PLLA nanofiber due to BTO nanoparticles. There were no differences in DNA amount among all nanofiber scaffold. ALP activity on PLLA/BTO nanofiber scaffold was higher than that on PLLA nanofiber scaffold. It was suggested that PLLA/BTO nanofiber scaffold enhanced osteogenic differentiation.

Free Research Field

生体医工学