Study for mechanism of biofilm formation on carbon steel surfaces

2014 Fiscal Year Final Research Report

• Project/Area Number: 24560914
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Metal making engineering
• Research Institution: Suzuka National College of Technology
• Principal Investigator: IKIGAI Hajime 鈴鹿工業高等専門学校, その他部局等, 教授 (60184389)
• Co-Investigator(Renkei-kenkyūsha): KANEMATSU Hideyuki 鈴鹿工業高等専門学校, 材料工学科, 教授 (10185952)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 緑膿菌 / バイオフィルム / 鉄イオン / 鉄鋼材料 / 炭素鋼 / 微生物腐食 / ３D可視化 / オートインデューサー

Outline of Final Research Achievements

In this study, we aimed to clarify the mechanisms of how Pseudomonas aeruginosa PAO1 (PAO1) induces biofilm formation and microbiologically influenced corrosion (MIC) on the surface of carbon steels. PAO1 biofilm was not formed on a surface of carbon steel which was treated to inhibit elution of ferrous ions by zinc phosphate coating, even if iron sulfate (Ⅱ) was included in the PAO1 culture. Thus, these data imply that ferrous ions eluted from the carbon steel are required for formation of PAO1 biofilm. In 3D-reconstruction of X-ray computed tomography images of the carbon steel after an incubation period 30 days with PAO1, it is clearly shown that MIC is caused directly under biofilm. We speculate that MIC might be caused by oxygen concentration cells which were formed by the uneven surface structure of biofilm.

Free Research Field

微生物学