2014 Fiscal Year Final Research Report
Thermal Stabilization Property of Proteins Adsorbed onto Biomass Charcoal Powder Prepared at Low Temperatures
| Project/Area Number |
24561013
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Recycling engineering
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| Research Institution | Tokyo Metropolitan University |
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Principal Investigator |
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| Research Collaborator |
ISHIYAMA Reona
IWAI Daiki
ENDO Nobuyuki
KAI Ryotaro
KATO Satoru
KURIHARA Shunichi
SATO Kunimichi
TAKEMOTO Tetsuya
TANAKA Masahiko
NISHIGAMI Junpei
HISHINUMA Keito
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 低温創製バイオマス炭 / タンパク質 / 熱安定化 / 吸着 / 固定化 / 残存活性 / リゾチーム / α-キモトリプシン |
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| Outline of Final Research Achievements |
The utilization of biomass for energies and functional materials is one of the most important challenges to establish a recycling society. We have found out that biomass charcoal powder (BCP), which is prepared from plant biomass waste by pyrolysis at low temperatures, is very useful as a carrier for the thermal stabilization of proteins. Proteins were effectively adsorbed onto BCP. The thermal stability of proteins adsorbed onto BCP was much greater than that of free proteins, and was strongly dependent upon the adsorption conditions such as solution pH, ionic strength, and temperature. Moreover, we have revealed the heat-resistant mechanism on the basis of the structure of proteins. On the other hand, the denaturation of proteins was efficiently inhibited in an organic solvent, which worked as a denaturant, by the adsorption of proteins onto BCP.
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| Free Research Field |
酵素工学
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