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2015 Fiscal Year Final Research Report

Structure and function of bacterial lipid domains

Research Project

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Project/Area Number 24570003
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Genetics/Genome dynamics
Research InstitutionSaitama University

Principal Investigator

MATSUMOTO Kouji  埼玉大学, 理工学研究科, 教授 (00119140)

Co-Investigator(Kenkyū-buntansha) HARA Hiroshi  埼玉大学, 理工学研究科, 准教授 (00173071)
MATSUOKA Satoshi  埼玉大学, 理工学研究科, 助教 (90509283)
Research Collaborator SADAIE Yoshito  
HASHIMOTO Michihiro  
KUSAKA Jin  
SHUTO Satoshi  
ISHIKAWA Kazuki  
IMAI Yukiko  
TANIGUCHUI Aya  
ITOU Aya  
MIYAGAWA Hiroyoshi  
UMEKAWA Mitsuru  
KONDO Daitetsu  
SEYA Manato  
MINESHIMA Ryota  
MIYAMATSU Saori  
MATSUSHIMA Wakana  
SEKI Takahiro  
NISHINO Yuki  
FURUKAWA Yugo  
SAITO Tomo  
NATORI Kohei  
Project Period (FY) 2012-04-01 – 2016-03-31
Keywords脂質ドメイン / カルジオリピン / ホスファチジルエタノールアミン / 枯草菌 / カルジオリピン合成酵素 / MinD / 膜結合配列
Outline of Final Research Achievements

To clarify the structure and function of bacterial lipid domains, we have adopted following two approaches. i) To elucidate the mechanism of formation of cardiolipin domain in Bacillus subtilis cells, the function of C-terminal α-helices of cardiolipin synthase is examined for septal membrane localization by fluorescence microscopy and Western blotting using GFP-ClsA fusion proteins. The enzyme is shown to be septally localized by means of its C-terminal α-helices, indicating that the C-terminal α-helices of the enzyme have a function of membrane targeting. ii) B. subtilis MinD is examined for septal localization in minJ mutant cells and is shown to be septally localized by means of the membrane targeting sequence at its C-terminus. This indicates that a correction in the current model of the sequential interaction for MinD binding to septal membranes is required.

Free Research Field

生物学分野 基礎生物学

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Published: 2017-05-10  

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