2014 Fiscal Year Final Research Report
Transcriptome analysis of two genetically distinct strains of Kanzawa spider mite to clarify the molecular mechanisms of host plant adaptation in the spider mite.
Project/Area Number |
24570023
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Ecology/Environment
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Research Institution | Kyoto University |
Principal Investigator |
OZAWA Rika 京都大学, 生態学研究センター, 研究員 (90597725)
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Co-Investigator(Kenkyū-buntansha) |
ARIMURA Genichiro 東京理科大学, 基礎工学研究科, 准教授 (60505329)
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Project Period (FY) |
2012-04-01 – 2015-03-31
|
Keywords | カンザワハダニ / トランスクリプトーム解析 / 解毒酵素 / 農薬抵抗性 |
Outline of Final Research Achievements |
Two strains of Kanzawa spider mite (KSM) - the white scar strain (W-strain) and the red scar strain (R-strain) - induce distinct defense response in the same Phaseolus leaves. In this study, we carried out RNAseq-based transcriptome analysis for mRNA of R-strain and W-strain on kidney bean plants (P. vulgaris) to identify KSM genes expressed specifically in either R-strain or W-strain. Of particular interest is the fact that some genes involved in the detoxification system in KSMs were expressed higher in R-strains than in W-strains. Some of detoxification enzyme activities were higher in R-strain than in W-strains as well. Further we investigated whether the differences in detoxification affect the tolerance of acaricides. R-strain showed less mortality than W-strain when they were treated with 3 out of 5 acaricides, suggesting that R-strain latently possesses higher detoxification ability than W-strain.
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Free Research Field |
化学生態学
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