Molecular interactions at plant-insect interface

2014 Fiscal Year Final Research Report

• Project/Area Number: 24570026
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ecology/Environment
• Research Institution: Okayama University
• Principal Investigator: GALIS Ivan 岡山大学, 資源植物科学研究所, 教授 (90360502)
• Co-Investigator(Kenkyū-buntansha): SHINYA Tomonori 岡山大学, 資源植物科学研究所, 助教 (80514207) ; TANI Akio 岡山大学, 資源植物科学研究所, 准教授 (00335621)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 防御応答 / 昆虫エリシター / 植食生昆虫 / ジャスモン酸 / Oryza sativa / ファイトアレキシン / イネ / 傷害

Outline of Final Research Achievements

We established a novel system to study rice-insect interactions. We showed that the accumulation of active defense hormone jasmonoyl-L-isoleucine (JA-Ile) in rice depends on the function of OsJAR1 enzyme. JA and JA-Ile accumulations in rice were controlled by the availability of the main pathway substrate, α-linolenic acid. In comparison to wounding, rice plants specifically responded to elicitors present in chewing herbivore oral secretions. We identified two novel herbivory-related phenolamide phytoalexins. Using ROS and rice phenolamides as molecular markers, we identified and partially purified a new putative insect elicitor from Mythimna loreyi oral secretions.

Free Research Field

生物学