Effects of phospholipid biosynthesis mutation on the mitochondrial respiratory activity at ambient and low temperatures

2014 Fiscal Year Final Research Report

• Project/Area Number: 24570040
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Saitama University
• Principal Investigator: NISHIDA Ikuo 埼玉大学, 理工学研究科, 教授 (40189288)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ホスファチジルエタノールアミン / ミトコンドリア内膜 / 呼吸活性 / シトクロムオキシダーゼ / 葉の発達 / 葉の呼吸活性 / リン脂質組成 / pect1-4

Outline of Final Research Achievements

Mitochondria are important organelles for respiratory activity in plants. In this study, the role of phosphatidylethanolamine, which is the major non-bilayer phospholipid of mitochondrial inner membrane, is investigated using Arabidopsis pect1-4 mutants, which exhibit one fourth of the CTP:phosphorylethanolamine cytidylyltransferase activity. We found that the leaf respiratory activity increases with rosette leaf development in the wild type, but it did not increase in pect1-4 mutants under prolonged short-day growth conditions. Cytochrome oxidase activity was selectively inhibited in pect1-4 mutants, with no change in COXII protein levels. In pect1-4 mutants, PE levels decreased in isolated mitochondria compared with that in wild-type mitochondrial. These results suggested that a shortage of phosphatidylethanolamine in the mitochondrial inner membrane causes a partial inhibition of COX activity.

Free Research Field

植物分子生理学