Characterization of plant stringent regulation by introducing artificial ppGpp-signal system

2014 Fiscal Year Final Research Report

• Project/Area Number: 24570054
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Saitama University (2014); Ehime University (2012-2013)
• Principal Investigator: TOZAWA Yuzuru 埼玉大学, 理工学研究科, 教授 (90363267)
• Co-Investigator(Kenkyū-buntansha): OGAWA Atsushi 愛媛大学, プロテオサイエンスセンター, 准教授 (30442940)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 葉緑体 / 翻訳制御 / 核酸代謝 / 緊縮制御 / ppGpp

Outline of Final Research Achievements

In this study, we have performed experiments for clarifying function of ppGpp in plant chloroplasts. By establishing in vitro translation system from isolated chloroplasts, we have demonstrated that chloroplast translation activity is inhibited by ppGpp. Further, we have shown that activity of guanylate kinase, a key enzyme for GTP biosynthesis in chloroplasts, is potently inhibited by ppGpp. We have thus proven that ppGpp regulates chloroplast translation and GTP biosynthesis in plants. Moreover, we have established cyanobacterial genetic system that enables induction of protein-expression by theophylline administration based on riboswithch system. The system has also been applied to transformation of tobacco chloroplast genome.

Free Research Field

分子生物学