The regulation of tubule formation in the endoplasmic reticulum of higher plant cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24570057
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: University of Hyogo
• Principal Investigator: YOKOTA Etsuo 兵庫県立大学, 生命理学研究科, 助教 (80212299)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 小胞体 / ＲＨＤ３ / リン酸化 / キナーゼ / フォスファターゼ / シロイヌナズナ

Outline of Final Research Achievements

We investigated the regulatory mechanism of RHD3, a fusogen of endoplasmic reticulum (ER) membrane. The fusion of ER membrane isolated from Arabidopsis seedling and cultured cell, and the oligomerization of RHD3 were enhanced by the treatment with protein kinases. The serine residues in the C-terminus serine cluster of RHD3 were phosphorylated. The membrane fusion enhancement did not occur with isolated ER from seedlings expressing the RHD3 with disrupted phosphorylation sites in the serine cluster. These results indicated that the phosphorylation of RHD3 regulates the ER membrane fusion.

Free Research Field

植物生理学