Application of a unique 3-D reconstruction software unaffected by the missing data range to biological tomography

2014 Fiscal Year Final Research Report

• Project/Area Number: 24570076
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Kogakuin University
• Principal Investigator: BABA Misuzu 工学院大学, 付置研究所, 研究員 (80435528)
• Co-Investigator(Renkei-kenkyūsha): BABA Norio 工学院大学, 情報学部, 教授 (80164896)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 電子顕微鏡 / 電子線ＣＴ / 酵母 / 急速凍結置換法 / 画像処理 / 位置合わせ / ニューラルネットワーク

Outline of Final Research Achievements

Electron CT is widely used as an efficient technique for analyzing three-dimensional intracellular membrane structures using electron microscopy. This is because three-dimensional observation enables us to capture the connection among membrane structures even though they appear to be separated on the two-dimensional images. At the present, for this technique, manual control in the foreign-made software is widely used. However, such manually operated work process is a major problem for many researchers that enormous energy and time are spent. In this study, we developed a new elemental technology to avoid such a problem. We succeeded to develop a software that enables automation and increase in speed for Electron CT analysis, resulting in reducing time and improvement of accuracy of the work.

Free Research Field

微細形態