2014 Fiscal Year Final Research Report
Structural basis for the post translational membrane insertion by GET complex
| Project/Area Number |
24570126
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 結晶構造解析 / テイルアンカー型タンパク質 |
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| Outline of Final Research Achievements |
Get3 is the specific targeting factor for the tail anchored proteins, which is defined by the C-terminal single transmembrane domain. After capturing the TA proteins, Get3 is further localized on the ER membrane through the interaction with Get1/Get2 membrane proteins. In this study, we succeeded to solve the crystal structure of the complex between Get3 and Get1 cytosolic domain. In addition, we succeeded to purify the Get3-TA complex coexpressed in E. coli. By using the mutant Get3, we succeeded to obtain the Get3-TA complex at a molar ratio of 2:1 (Get3 dimer bound to single TA). Further, we succeeded to purify Get1, Get2 using insect cell expression system.
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| Free Research Field |
構造生物化学
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