Conformational change of photoreceptor membrane protein using by in-situ photo-irradiated solid-state NMR

2014 Fiscal Year Final Research Report

• Project/Area Number: 24570127
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Yokohama National University
• Principal Investigator: KAWAMURA Izuru 横浜国立大学, 工学(系)研究科(研究院), 准教授 (20452047)
• Co-Investigator(Renkei-kenkyūsha): NAITO Akira 横浜国立大学, 大学院工学研究院, 教授 (80172245)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 固体NMR / レチナール / In-situ光照射 / センサリーロドプシンI / 膜タンパク質 / バクテリオロドプシン

Outline of Final Research Achievements

In-situ photo-irradiated solid-state NMR system: An optical fiber is guided from the outside of the magnet and illumination the light to the top of the spinner. The light (365, 520 or 595 nm) is penetrated from the top part of the rotor into the sample tube without any contact, so that the rapid magic angle spinning was successfully established. Using this NMR apparatus, we could efficiently irradiate light to photoreceptor membrane protein samples. We firstly observed 13C NMR signals of retinal in M (positive phototaxis) and P (negative phototaxis) photo-intermediates of SRI from Salinibacter ruber. Interestingly, we also record that ground-state directly transformed itself into P-intermediate when ground-state absorbed blue light without green light irradiation to accumulate M intermediate. Our NMR results provide insights into the color-discriminating retinal-configurations in SrSRI.

Free Research Field

構造生物化学