2014 Fiscal Year Final Research Report
Role of the glycolipid receptor in the digestive tract and bacterial glycolipid antigen in bacterial infection and symbiosis
| Project/Area Number |
24570141
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kinki University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 共生細菌 / グリセロ糖脂質 / スフィンゴ糖脂質 / 自然抗体 / TLC-免疫染色 / 細菌受容体糖脂質 / GA1のフコシル化 / ABO血液型ヒト血清 |
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| Outline of Final Research Achievements |
Intestinal lactobacilli in immune-deficient scid mice were distinct from those in control mice, i.e. L. murinus (LM) for scid and L. johnsonii (LJ) for control, respectively, which were closely related with the loose and solid properties of the caecal and colonal contents of scid and control mice, respectively. LJ contained LacTH-DG and LacTetH-DG, both of which were modified LacDH-DG with Galα1-6 linkage, whereas, LM contained LacβTH-DG modified LacDH-DG with Glcβ1-6 linkage. Since natural antibodies toward LacTH-DG and LacTetH-DG were frequently detected in human sera, irrespective of ABO-blood groups, in the similar intensities as those toward blood groups- and species-specific glycolipids, immune system is involved in the establishment of symbiotic relationship with bacteria in the intestine. On the other hand, enhanced fucosylation of GA1 as a receptor for LJ occurred in the digestive tracts of scid mice, probably to interfere the formation of bacterial flora of LJ by LM.
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| Free Research Field |
生物学
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