Development of nucleic acids therapeutics against multiple domains of transcription factor SATB1

2014 Fiscal Year Final Research Report

• Project/Area Number: 24570144
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: YAMASAKI Kazuhiko 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (00358243)
• Co-Investigator(Renkei-kenkyūsha): MIYAGISHI Makoto 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 研究グループ長 (30323538) ; OKADA Tomoko 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 上級主任研究員 (30344146)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 転写因子 / 核酸創薬 / がん / タンパク質核酸相互作用

Outline of Final Research Achievements

The scope of this project is to obtain nucleic acids that have inhibitory activities against transcription factor SATB1 through binding to its functional domains. We investigated properties of three DNA-binding domains by isothermal titration calorimetry and found that only one of them (CUTr1) is responsible for the sequence-specific DNA binding. By introducing phosphorothioate modification, we obtained a high-affinity nucleic acid especially under salt concentration close to biological condition. Crystal structure of complex of this nucleic acid and CUTr1 revealed that hydrophobic interactions between phosphorothioate and amino acid side chain are responsible for the high affinity.

Free Research Field

構造生物学、生化学