2014 Fiscal Year Final Research Report
Analysis of the effects of lipid environment and roles of electrostatic interactions on the Ca2+-ATPase function
| Project/Area Number |
24570147
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | カルシウムポンプ / Ca2+-ATPase / 酵素反応 / 生体エネルギー転換 / 静電的相互作用 / ナノディスク / リン脂質 / タンパク-脂質相互作用 |
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| Outline of Final Research Achievements |
The role of electrostatic interactions in and the effects of the lipid environment on the reaction mechanisms on sarcoplasmic reticulum Ca2+-ATPase were explored. The experiments using the Ca2+-ATPase buried into nanodiscs which contained various lipid contents revealed that the not only electrostatic interactions between protein and lipids but also steric interactions between them have important for function of the pump. This study succeeded clearly to divide the effects of the lipid environment between electrostatic interactions and steric interactions.
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| Free Research Field |
筋小胞体カルシウムポンプの構造機能連関
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