2014 Fiscal Year Final Research Report
Study on the epiginetic silencing of diacylglycerol kinase gamma in colorectal cancer
Project/Area Number |
24570166
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Functional biochemistry
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Research Institution | Sapporo Medical University |
Principal Investigator |
KAI Masahiro 札幌医科大学, 医学部, 講師 (80260777)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
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Keywords | 大腸がん / エピジェネティクス / ジアシルグリセロールキナーゼ |
Outline of Final Research Achievements |
In attempt to search the genes repressed in colorectal cancer-derived cell lines, we found that DGKG expression is strongly suppressed in the cancer cells from both of cell lines and primary tumors. Because DGKG contains a CpG island in the region around its transcription start site, we carried out bisulfite pyrosequencing analysis, resulting that DGKG is strongly methylated in colorectal cancer cells. These findings confirmed the presence of high levels of methylation in most of the cells in which DGKG expression was silenced. The overexpression of DGKG in a DGKG-silenced colorectal cancer cell line, HCT116 decreased the migration and invasion activities of the cells significantly. In addition, DGKG activity modified the activation levels of Rac1 and Rho small GTPase proteins. These results suggested that DGKG regluated cell migration via Rho-family small GTPases. The epigenetic silencing of DGKG may correlate with the cancer malignancy in colorectal cancer.
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Free Research Field |
分子生物学
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