2014 Fiscal Year Final Research Report
Generation and functional analysis of monoclonal antibodies raised against human iPS cells
| Project/Area Number |
24570171
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | Ritsumeikan University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAWASAKI Nobuko 立命館大学, 総合科学技術研究機構, 教授 (70077676)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Keywords | 多能性幹細胞 / 糖鎖認識抗体 / ケラタン硫酸 / 糖脂質 / ES細胞 / iPS細胞 |
|---|
| Outline of Final Research Achievements |
In this study, we generated two hybridomas using a hiPS cell line (Tic) as an antigen. These hybridomas produced antibodies that specifically bound to hiPS/ES cells but exhibited little or no binding to hEC cells. Interestingly, both of these antibodies were found to be carbohydrate–recognizing antibodies of the IgG1 subtype. 1st antibody R-10G recognizes a type of keratan sulfate lacking oversulfated structures expressed on podocalyxin molecules on hiPS/ES cells like anti-TRA-1-60 antibody. 2nd antibody R-17F recognized glycolipids like anti-SSEA-3 antibody, and exhibited a strong cytotoxic effect toward hiPS/ES cells.
|
| Free Research Field |
糖鎖生物学
|
