2014 Fiscal Year Final Research Report
Characterization of biosynthesis and regulation mechanisms of sufur-modification in tRNA
| Project/Area Number |
24570173
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
SHIGI Naoki 独立行政法人産業技術総合研究所, 創薬基盤研究部門, 主任研究員 (20392623)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 核酸 / タンパク質 / tRNA / RNA転写後修飾 / 酵素 / 硫黄転移反応 / タンパク質翻訳後修飾 / 好熱菌 |
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| Outline of Final Research Achievements |
Post-translaional modification of RNA is one of the important features of RNA molecules. In this study, the biosynthesis pathway of thiouridine found in tRNA of thermophilic bacteria was analyzed. An in vitro reconstitution system for tRNA thiolation reaction suitable for the quantitative analysis was constructed. By using this system and information of the three-dimensional structure of the thiolation enzyme, a novel reaction mechanism for the thiolation reaction was suggested.
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| Free Research Field |
分子生物学・生化学
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