2014 Fiscal Year Final Research Report
Regulation of Bach2 intrinsically disordered region by ligand
| Project/Area Number |
24570175
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MUTO Akihiko 東北大学, 大学院医学系研究科, 准教授 (80343292)
MATSUI Miki (WATANABE Miki) 東北大学, 大学院医学系研究科, 助教 (00455784)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | タンパク質構造変化 / 天然変性タンパク質 / ヘム結合 |
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| Outline of Final Research Achievements |
Bach2 plays an important role in the transcriptional process. Bach2 includes long intrinsically disordered regions. We prepared the region of 331-520 to investigate its biophysical properties. Spectroscopic measurements revealed that Bach2(331-520) is intrinsically disordered region, in which heme binds. SAXS and dynamic light scattering mesurements indicated that heme binding form of Bach2(331-520) is unstabler than the non heme binding form and the structure will be more compact. Our results showed the first example of the heme binding protein which is (1) intrinsically disordered protein/region, (2) indicates structural change by heme binding.
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| Free Research Field |
生物物理化学
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