Effect of amyloid aggregation on dynamic properties of lipid membranes

2014 Fiscal Year Final Research Report

• Project/Area Number: 24570185
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biophysics
• Research Institution: Kobe University
• Principal Investigator: SASAHARA KENJI 神戸大学, 医学(系)研究科(研究院), 研究員 (20432495)
• Co-Investigator(Kenkyū-buntansha): MORIGAKI Kenichi 神戸大学, 遺伝子実験センター, 准教授 (10358179)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: アミロイド / 脂質膜

Outline of Final Research Achievements

We created the experimental methodology using supported planner lipid bilayers to observe the membrane binding of two peptides (islet amyloid polypeptide and amyloid beta-peptide) and their membrane-mediated amyloid aggregation. In the experimental system, the changes of membrane fluidity were quantitatively estimated for the membrane binding and subsequent aggregation of the peptides. Furthermore, it was found that the raft components of lipids were incorporated into the aggregates that deposited into the membranes in which phase separation of the raft components were involved.

Free Research Field

生物学