Molecular mechanism of tumor suppression mediated by the transcription factor E2F

2014 Fiscal Year Final Research Report

• Project/Area Number: 24570202
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Kwansei Gakuin University
• Principal Investigator: OHTANI Kiyoshi 関西学院大学, 理工学部, 教授 (30201974)
• Co-Investigator(Renkei-kenkyūsha): NAKAYAMA Jun-ichi 名古屋市立大学, システム自然科学研究科, 准教授 (60373338)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 癌 / 発現制御 / E2F / RB / がん化抑制

Outline of Final Research Achievements

The transcription factor E2F is the main target of the tumor suppressor RB. E2F plays central role in cell growth upon growth stimulation. E2F also plays important role in tumor suppression by inducing apoptosis or cellular senescence upon dysfuncrion of RB. We analyzed molecular mehcanism of discrimination of target genes involved in cell growth and tumor suppression, and identified involvement of phosphorylation status of E2F1. We searched new target genes specifically activatived by dysfunction of RB by using DNA microarray, and identified 9 novel targets including BIM. We searched new interacting proteins of E2F1 by using co-immunoprecipitation coupled with mass spectrometry and yeast two-hybrid system, and identified 52 candidates including DDX5.

Free Research Field

分子細胞生物学