Molecular mechanism of regulation of mitotic chromosome dynamics by condensins

2015 Fiscal Year Final Research Report

• Project/Area Number: 24570204
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Kinoshita Kazuhisa 国立研究開発法人理化学研究所, 平野染色体ダイナミクス研究室, 専任研究員 (60447886)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 染色体 / 細胞周期 / 細胞分裂

Outline of Final Research Achievements

Condensin complexes play an essential role in regulation of mitotic chromosome organization in eukaryotic cells. Using an experimental system to reconstitute recombinant condensin complexes in vitro, we analyzed the molecular functions of condensin I. We found that ATP binding and hydrolysis by SMC subunits have distinct contributions to condensin I’s action, and that continuous ATP hydrolysis is required for structural maintenance of chromosomes as well. We also found that balancing acts of two non-SMC subunits containing HEAT repeats support dynamic assembly of chromosome axes.

Free Research Field

分子生物学　細胞生物学