2014 Fiscal Year Final Research Report
Roles of the organelle-specific ubiquitination regulators in protein degradation
| Project/Area Number |
24570209
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | ユビキチン / タンパク質分解 / 小胞体 / ミトコンドリア |
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| Outline of Final Research Achievements |
Although protein quality control is essential for maintaining cellular function, its molecular mechanism is not fully understood. In this study, we performed the characterization of the endoplasmic-reticulum (ER) deubiquitinating enzyme USP19 and showed that USP19 stabilizes the ER-associated ubiquitinating enzyme MARCH6 via deubiquitination. It is known that MARCH6 has a role in protein quality control in the ER. In addition, the stabilization of MARCH6 increases its enzymatic activity and thereby promotes degradation of the mutant ABCB11, a substrate of MARCH6. These results suggest that USP19 is involved in the regulation of protein quality control in the ER.
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| Free Research Field |
細胞生物学
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