Function and dynamics of dynamin family in cytokinesis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24570218
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Yamaguchi University
• Principal Investigator: YUMURA shigehiko 山口大学, 医学(系)研究科(研究院), 教授 (70183986)
• Co-Investigator(Renkei-kenkyūsha): UYEDA Taro 産業総合研究所, バイオメディカル部門, 総括研究主幹 (90356551)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ダイナミン / 細胞質分裂 / アクチン / ミオシン / 細胞性粘菌

Outline of Final Research Achievements

Dynamin is an important protein to cut the vesicles from the cell membrane during endocytosis. Recently, dynamin has shown to play an important role on cytokinesis, which is a final stage of cell division. Inhibitors against dynamin, which can be used for targeting cytokinesis of cancer cells, are new potent and attractive anti-cancer reagents. This study clarified the function and dynamics of dynamin in cytokinesis of cellular slime mold cells, a model organism for cytokinesis. Dynamin null cells showed severe defects in cytokinesis, resulting in their multinucleation. Actin and myosin II could not recruit at the cleavage furrow properly. GFP-tagged dynamins accumulated to the cleavage furrow. We found that dynamins contribute to stabilization of actin in the contractile ring. Together, we proposed a new model for the role of dynamin in cytokinesis.

Free Research Field

分子細胞生物学