Origin and multipotency of Oct60-expressing cells in the adult tissue of Xenopus laevis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24570240
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Rikkyo University
• Principal Investigator: KINOSHITA TSUTOMU 立教大学, 理学部, 教授 (30161532)
• Co-Investigator(Kenkyū-buntansha): KUBO Hideo 東京都医学総合研究所, ゲノム医科学研究部門, 研究員 (50178034) ; MORICHIKA Keisuke 立教大学, 理学部, ポストドクトラルフェロー (30593074)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: アフリカツメガエル / 器官形成 / 多能性細胞 / Oct60

Outline of Final Research Achievements

Oct60 gene is Xenopus paralog of mammalian Oct3/4 gene which encodes a transcription factor of POU family class V. In immunohistochemical analysis, the Oct60-expressing cells were detected in adult gonads, liver, kidney and blood cells. The Oct60-expressing cells were stained with anti-Bmi1 antibody and increased in number by anemic induction, suggesting that the Oct60-expressing cells are hematopoietic precursor-like cells. Cell transplantation experiment indicated that the Oct60-expressing cells are derived from dorsolateral mesoderm of tail-bud stage embryos. Functional analysis of Oct60 showed that the Oct60-expressing cells are multipotent undifferentiated cells.

Free Research Field

生物学