2014 Fiscal Year Final Research Report
Identification of gamete fusion-related factors that are present on the oocyte surface
| Project/Area Number |
24570241
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Setsunan University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 精子 / 卵子 / 配偶子間融合 / 受精 / 線虫 / 免疫グロブリン様タンパク質 / 膜タンパク質 |
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| Outline of Final Research Achievements |
My laboratory has recently identified the C. elegans male-specific gene spe-45 that is essential for gamete fusion. In order to identify sperm or oocyte factors that associate with SPE-45 protein, I constructed a transgene encodong SPE-45 in which a FLAG-tag is inserted into the cytoplasmic tail domain. As the transgene was expressed in spe-45 mutants, self-fertility of the mutant worms was rescued to about 70% levels of wild-type worms. Currently, by using anti-FLAG antibody, I am trying to identify SPE-45-associating proteins.
I also tried to identify oocyte factors that are indispensable for gamete fusion by different ways. From the database, I selected oocyte-specific genes encoding transmembrane proteins. Then, I disrupted some of the selected genes by the CRISPR/Cas9 method. So far I have obtained five mutant strains, but neither of them were sterile at all. I'll continue to create mutants by the same way.
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| Free Research Field |
分子細胞生物学
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