MHC-linked microsatellite DNA: development of its potential as fitness-related molecular markers in captive breeding colonies of macaques and their application to a phylogeography study

2014 Fiscal Year Final Research Report

• Project/Area Number: 24570253
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical anthropology
• Research Institution: Kyoto University
• Principal Investigator: TANAKA Hiroyuki 京都大学, 霊長類研究所, 助教 (20335243)
• Co-Investigator(Kenkyū-buntansha): KAWAMOTO Yoshi 京都大学, 霊長類研究所, 准教授 (00177750)
• Co-Investigator(Renkei-kenkyūsha): HAMADA Yuzuru 京都大学, 霊長類研究所, 教授 (40172978)
• Research Collaborator: KAWAMOTO Sakie 京都大学, 霊長類研究所, 技能補佐 ; HIGUCHI Shoko 京都大学, 霊長類研究所, 技術補佐 ; MORIMOTO Mayumi 京都大学, 霊長類研究所, 技術職員
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 主要組織適合抗原複合体 / マイクロサテライトDNA / マカクザル / 繁殖集団 / ニホンザル / アカゲザル / ブタオザル種群 / 系統地理学

Outline of Final Research Achievements

In order to utilize information on major histocompatibility complex (MHC) genes into genetic management of the captive breeding colonies of Japanese (Macaca fuscata) and rhesus (M. mulatta) macaques, polymorphisms of microsatellites linked to MHC genes (MHC-MS) were examined. The haplotypes of the eight MHC-MS loci were first determined in Japanese macaques from the results of pedigree analyses. Although no direct association was found between MHC-MS alleles and disease in macaques died of illness, it will be important to genotype MHC-MS loci, as fitness-related markers, to the genetic management of breeding colonies. Finally, polymorphism of MHC-MS was examined in two species of the M. nemestrina group (M. leonina and M. nemestrina). The results were compared with mtDNA phylogeography and suggested that the mtDNA introgression between local populations of leonina and nemestrina was involved in phylogeographic history of the M. nemestrina group.

Free Research Field

動物遺伝学