Analysis of induction mechanism for ascorbic acid accumulation as antiviral reaction

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Breeding science
• Research Institution: Hokkaido University
• Principal Investigator: INUKAI Tsuyoshi 北海道大学, (連合)農学研究科(研究院), 講師 (90223239)
• Co-Investigator(Renkei-kenkyūsha): MASUTA Chikara 北海道大学, 大学院農学研究院, 教授 (60281854)
• Research Collaborator: FUJIWARA Ayaka
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: アスコルビン酸 / ウイルス / 抵抗性

Outline of Final Research Achievements

In this study, we first showed that endogenous ascorbic acid (AsA) functioned as an antiviral chemical to TuMV in Brassica rapa and Arabidopsis thaliana. Next, it was shown that the AsA accumulation was induced by TuMV infection in the B. rapa resistant cultivar Aki-masari. This AsA accumulation was due to suppression of AsA oxidation by ascorbate peroxidase (APX) and ascorbate oxidase and activation of AsA recycling by dehydroascorbate reductase (DHAR). Because methyl jasmonate (MeJA) suppressed the APX activity and enhanced the DHAR activity; JA derivatives including JA-isoleucine, tuberonic acid and tuberonic acid glucoside were increased together with AsA, the induction of the AsA accumulation in Aki-masari appeared to be mediated by the JA-dependent signaling pathway suggesting that the AsA accumulation was induced as a defense reaction against virus.

Free Research Field

植物育種学