Isolation and characterization of modifier locus for clubroot resistance in Brassica rapa.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580015
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Breeding science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: HATAKEYAMA Katsunori 独立行政法人農業・食品産業技術総合研究機構, 野菜茶業研究所 野菜育種・ゲノム研究領域, 主任研究員 (60355625)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ハクサイ / 根こぶ病 / 抵抗性遺伝子 / DNAマーカー

Outline of Final Research Achievements

We tested for clubroot resistance using F3 plants derived from the selected F2 plants with recombination in the 18-kb Crr2 candidate region. Crr2 was estimated to lie within 5.9-kb region based on the relationship between the genotype of markers within the candidate region and clubroot resistance. Two ORFs, ORF3 and ORF4, were speculated in this region. In the susceptible genome, a 1.6-kb insertion was found in 3’ UTR of ORF3 and a 591-bp deletion in the region including 5’ UTR, exon1 and a part of exon2 of ORF4. RT-PCR analysis revealed that ORF3 expressed in leaves of both resistant and susceptible lines whereas ORF4 did not expressed in leaves of the susceptible line. Transformation of ORF3 and ORF4 constructs into Brassica rapa is in progress.

Free Research Field

植物育種学