Development of efficient production methods for periclinal chimeric plants

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580056
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Horticulture/Landscape architecture
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: AIDA Ryutaro 独立行政法人農業・食品産業技術総合研究機構, 花き研究所花き研究領域, 上席研究員 (60355716)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 周縁キメラ / キク / 蛍光タンパク質遺伝子

Outline of Final Research Achievements

Production methods for periclinal chimeric plants on chrysanthemum (Chrysanthemum morifolium) and torenia (Torenia fournieri) were examined. Leaf explants of wild-type plants and transgenic plants having a fluorescent protein gene were placed on medium close to each other, and detached just after they partially combined. After 1 week culture on medium with an antibiotics for selection, mosaic calli consisted of transgenic cells and wild-type cells were formed on the edge of explants. Regenerated shoots (chrysanthemum 996, torenia 357) were analyzed by observation of fluorescence to detect chimeric structure. No chimeric plants was obtained on torenia, but a single sectorial- chimeric shoot was obtained in chrysanthemum. Chrysanthemum plants of L1 periclinal chimera and L3 periclinal chimera were obtained from the sectorial-chimeric shoot after repeated axillary bud elongation.

Free Research Field

花卉園芸学