Taxonomic study of a Japanese native grape cultivar 'Koshu' through SNPs analysis and partial sequence of chloroplast DNA

2015 Fiscal Year Final Research Report

• Project/Area Number: 24580063
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Horticulture/Landscape architecture
• Research Institution: National Research Institute of Brewing
• Principal Investigator: Goto Nami 独立行政法人酒類総合研究所, 役員, 理事 (60372190)
• Research Collaborator: Sawler Jason Dalhousie University ; Myles Sean Dalhousie University
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: ブドウ / 甲州 / SNP / 葉緑体DNA / Vitis vinifera

Outline of Final Research Achievements

‘Koshu’ is a Japanese native grape cultivar and had been believed to be an oriental variety of Vitis vinifera; however, there were different opinions. Thus, to clarify the taxonomic position of ‘Koshu’, we carried out polymorphic DNA analyses. The result of single nucleotide polymorphisms (SNPs) analysis indicated that genomic DNA of ‘Koshu’ consists of slightly more than 70% of V. vinifera and slightly less than 30% of Asian wild species. In addition, the chloroplast DNA, which is inherited from the maternal ancestor, of ‘Koshu’ is a wild type and most closely related to that of a Chinese wild speciesV. davidii. From these results, it was revealed that ‘Koshu’ is a hybrid cultivar with V. davidii or closely related species as the maternal ancestor, even though the percentage of V. vinifera is higher than that of wild species.

Free Research Field

醸造学