Analysis of conferred disease resistance by artificial control of phosphatidc acid metabolism in plants

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580066
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Kochi University
• Principal Investigator: KIBA Akinori 高知大学, 教育研究部総合科学系, 教授 (50343314)
• Co-Investigator(Kenkyū-buntansha): HIKICHI Yasufumi 高知大学, 教育研究部総合科学系, 教授 (70291507) ; OHNISHI Kouhei 高知大学, 教育研究部総合科学系, 教授 (50211800)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 植物 / 免疫 / フォスファチジン酸 / 耐病性

Outline of Final Research Achievements

DS1 plants that showed accelerated defense responses in response to compatible Ralsto nia solanacearum infection. We analyzed activation mechanisms of defense responses in DS1 plants.DS1 plants showed hyper induction of hypersensitive response and reactive oxygen species generation. Acceleration of HR and ROS was closely associated with enhanced phosphatidic acid contents. We created DS1-knockdown transgenic tobacco, that showed resistance against R. solanacearum. We also created DS1-knockdown transgenic tomato. In addition, we screened DS1-deficient mutant of Arabisopusis. Therefore, we will analyze immune response using DS1-knockdown tobacco and tomato, and DS-feficient Arabidopsis. In addition, we successfully isolated promoter region of DS1 gene, and we started promoter analysis of DS1 gene.

Free Research Field

植物病理学