Molecular mechanisms of synergism between plant viruses

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580067
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Kyushu University
• Principal Investigator: TAKESHITA Minoru 九州大学, (連合)農学研究科(研究院), 助教 (00304767)
• Co-Investigator(Kenkyū-buntansha): 古屋 成人 九州大学 (10211533) ; 土屋 健一 九州大学 (40150510)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: CMV / TuMV / synergism / サリチル酸 / 2bタンパク質

Outline of Final Research Achievements

Spatial interactions between Cucumber mosaic virus (CMV) and Turnip mosaic virus (TuMV) were analyzed in Nicotiana benthamiana for better understand of the molecular mechanisms associated with local interference which can be observed before establishment of plant viral synergism. CMV and TuMV vectors expressing fluorescent protein DsRed2 (Ds) and E-GFP (EG) were used for inoculation onto N. benthamiana, respectively. A1 vector (A1) has a functional 2b while H1 vector (H1) is 2b-deficient. Curiously, a combination of H1Ds and TuEG compromised local interference in N. benthamiana nahG transgenic line. The results suggested that the SA-mediated host resistance and the 2b protein of CMV are involved in local interference.

Free Research Field

植物病理学