Study of suppression mechanisms of anti-viral PTGS of host plant in Cucumber mosaic virus infection

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580069
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Osaka Prefecture University
• Principal Investigator: OHKI Satoshi T. 大阪府立大学, 生命環境科学研究科(系), 教授 (00128761)
• Co-Investigator(Renkei-kenkyūsha): MOCHIZUKI Tomofumi 大阪府立大学, 生命環境科学研究科, 助教 (30469837)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: キュウリモザイクウイルス / ウイルスサプレッサー / PTGS / VIGS

Outline of Final Research Achievements

Effects of Cucumber mosaic virus (CMV) pepo strain infection on post-transcriptional gene silencing (PTGS) in host plants were investigated. To distinguish the primary and the secondary PTGS, we prepared transgenic tobacco carrying GFP mRNA and GF (5′ two-third region of GFP gene) inverted-repeat constructs; siRNA from P region (3’ one-third region of GFP gene) that was indicator of secondary PTGS was not detected, suggesting that the secondary PTGS was not induced toward 3′ region of the GFP mRNA. We also constructed mutated CMV that had the 30 nt GFP sequence. Inoculation of the mutated CMV to the GFP expressing transgenic tobacco showed virus-induced gene silencing (VIGS) of GFP. Secondary VIGS in the 5′ region of GFP was observed but not in the 3′ region. Finally, we examined the effect of CMV pepo infection on endogenous PTGS, such as miRNA and ta-siRNA, of Arabidopsis. CMV pepo infection increased the expression of mRNAs that were targets of miRNA or ta-siRNA.

Free Research Field

農学