Construction of a base for utilization of insect cell-free protein synthesis system

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580083
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Okinawa National College of Technology
• Principal Investigator: ITO Masaaki 沖縄工業高等専門学校, 生物資源工学科, 教授 (00395659)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 無細胞タンパク質合成系 / 昆虫 / カイコ / 翻訳促進配列 / チロシナーゼ / ラッカーゼ

Outline of Final Research Achievements

In the present study, we analyzed an enhancer sequence composed of Spodoptera frugiperda nucleopolyhedrovirus polyhedorin 5’-UTR for improvement of Bombyx mori cell-free protein synthesis system. As a result, we found important sequence of 18 base length composed 3'-terminal region. A protein synthesis activity using the sequence as enhancer was 1.3-fold greater than that of using usual enhancer.
We constructed cell-free translation and analysis system with co-expression of activation protein using organic solvent resistant tyrosinase as a model protein. Furthermore, we also constructed membrane-binding protein expression and analysis system using thermostable laccase as a model protein.

Free Research Field

タンパク質工学