Basic research for accumulating oil in plant vegetative tissues

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580091
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: SHIMOJIMA Mie 東京工業大学, バイオ研究基盤支援総合センター, 助教 (90401562)
• Co-Investigator(Renkei-kenkyūsha): 太田 啓之 東京工業大学, バイオ研究基盤支援総合センター, 教授 (20233140)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 植物脂質 / 貯蔵脂質 / トリアシルグリセロール / リン欠乏

Outline of Final Research Achievements

We produced Arabidopsis transgenic plants overexpressing one of the key enzymes in TAG synthesis (diacylglycerol acyltransferase 1, 2 and phospholipid: diacylglycerol acyltransferase 1) during phosphate starvation by utilizing a phosphate-deficiency responsive promoter (AtMGD3 promoter) using wild-type or pgm-1 as genetic backgrounds for transformation. The produced transgenic plants accumulated large amount of oil in shoots under phosphate-depleted conditions, whereas the effect was different from the results obtained from roots. From these results, it was suggested that the mechanism of oil accumulation during phosphate starvation was different between shoots and roots.

Free Research Field

植物生理学