Functional analysis of transporters regulating translocation of energy metabolites in rice organelle

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580094
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Ehime University
• Principal Investigator: NOZAWA Akira 愛媛大学, プロテオサイエンスセンター, 講師 (30432800)
• Co-Investigator(Renkei-kenkyūsha): TOZAWA Yuzuru 埼玉大学, 大学院・理工学研究科, 教授 (90363267)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 糖リン酸輸送体 / ミトコンドリアキャリア / コムギ無細胞系 / リポソーム

Outline of Final Research Achievements

We improved a method for functional analysis of transporter proteins based on liposome-supplemented cell-free system. In the new system, detection range of transport activity against non-specific background activity was improved by up-regulated synthetic efficiency of membrane proteins in cell-free reaction. By using this method, we analyzed several transporter proteins localized in plant organelle. Substrate specificity of sugar-phosphate transporters in rice and iceplant plastids and sulfate metabolite transporter in Arabidopsis plastids.

Free Research Field

植物栄養学・土壌学