Development of a Corynebacterium glutamicum strain with an NADPH-generating glycolytic pathway

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580105
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Shinshu University
• Principal Investigator: IKEDA Masato 信州大学, 学術研究院農学系, 教授 (00377649)
• Co-Investigator(Kenkyū-buntansha): TAKENO Seiki 信州大学, 学術研究院農学系, 助教 (30422702)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: コリネバクテリウム・グルタミクム / 還元力 / NADPH / GapN / リジン発酵

Outline of Final Research Achievements

Streptococcus mutans, a tooth decay bacterium, has an unusual glycolytic pathway that can generate NADPH at the step of glyceraldehyde 3-phosphate dehydrogenase. We engineered Corynebacterium glutamicum with an S. mutans-type NADPH-generating glycolytic pathway. A lysine producer derived from the engineered strain produced more than twice as much lysine as the reference strain. The production of lysine remained high after the pentose phosphate pathway was interrupted, while a drastic decrease in lysine production was observed in the case of the reference strain.

Free Research Field

応用微生物学