2014 Fiscal Year Final Research Report
Analysis of Photoresponse Genes of Mushroom Mycelia and Correlation with Primary Metabolites
| Project/Area Number |
24580106
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
FUJII Hiroshi 信州大学, 学術研究院農学系, 教授 (90165340)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 糸状菌の光応答 / 青色光刺激 / シキミ酸蓄積 / 遺伝子発現制御 / タンパク質発現制御 / 新規有用物質生産法の開発 / 一次代謝経路の光制御 / 発光ダイオード |
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| Outline of Final Research Achievements |
Shikimic acid is a key intermediate in the aromatic amino acid pathway as well as an important starting material for the synthesis of Tamiflu, a potent and selective inhibitor of the neuraminidase enzyme of influenza viruses A and B.
In oyster mushroom mycelia cultivated in the dark, stimulation with blue light-emitting diodes induces the accumulation of shikimic acid. An integrated analysis of primary metabolites, gene expression and protein expression suggests that the accumulation of shikimic acid caused by blue light stimulation is due to an increase in 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase (DAHPS), the rate-determining enzyme in the shikimic acid pathway, as well as phosphofructokinase and glucose-6-phosphate dehydrogenase, the rate-determining enzymes in the glycolysis and pentose phosphate pathways, respectively. This stimulation results in increased levels of phosphoenolpyruvic acid and erythrose-4-phosphate, the starting materials of shikimic acid biosynthesis.
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| Free Research Field |
光生物科学
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